Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan
- 標題
- Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan
- 作者
- Yuji SHIBATA 1, Katsuhisa YAMADA 2, Chiaki IMADA 1, Takeshi KOBAYASHI 1 and Takeshi TERAHARA 1
- 文件屬性
- 國外期刊
- 知識分類
- 基礎研究
- 關鍵字
- Deep seawater, Fibroblast, Pseudoalteromonas denitri cans, Oxidative stress resistance
- 點閱數
- 2834
摘要
Abstract
In this study, we tried to isolate microbes from a bag lter (BF) which was used to remove suspended solids from deep seawater (DSW) at a DSW pumping station in Izu-Akazawa, Shizuoka
prefecture, Japan. As a result, 941 strains were isolated and only 10 strains from all the isolates showed a resistant e ect against oxidative stress. The one strain which showed the highest ability
of oxidative stress resistance among 10 strains was called strain “No. 586”, and its 16S rRNA sequence analysis was performed. As a result, the homology of strain No. 586 matched
Pseudoalteromonas denitri cans JCM21248 at 98.07％. After comparing the phenotypic characteristics of strain No. 586 and the type strain, it was shown that there were di erences in the growth
temperature and the color of the colony between the two strains. Furthermore, the resistant e ect of strain No. 586 against oxidative stress was remarkably higher than that of the type strain.
In this study, we tried to isolate microbes from a bag lter (BF) which was used to remove suspended solids from deep seawater (DSW) at a DSW pumping station in Izu-Akazawa, Shizuoka
prefecture, Japan. As a result, 941 strains were isolated and only 10 strains from all the isolates showed a resistant e ect against oxidative stress. The one strain which showed the highest ability
of oxidative stress resistance among 10 strains was called strain “No. 586”, and its 16S rRNA sequence analysis was performed. As a result, the homology of strain No. 586 matched
Pseudoalteromonas denitri cans JCM21248 at 98.07％. After comparing the phenotypic characteristics of strain No. 586 and the type strain, it was shown that there were di erences in the growth
temperature and the color of the colony between the two strains. Furthermore, the resistant e ect of strain No. 586 against oxidative stress was remarkably higher than that of the type strain.