富山湾深層水からのEnterococcus 属乳酸菌の分離と諸性状の検討(Isolation and phenotypic characterization of enterococci from the deep-seawater samples collected in Toyama Bay)
- 標題
- 富山湾深層水からのEnterococcus 属乳酸菌の分離と諸性状の検討(Isolation and phenotypic characterization of enterococci from the deep-seawater samples collected in Toyama Bay)
- 作者
- 林 篤志, 嶋田 貴志, 尾仲 宏康, 古米 保(Atsushi HAYASHI, Takashi SHIMADA, Hiroyasu ONAKA, Tamotsu FURUMAI)
- 文件屬性
- 日本研究
- 知識分類
- 基礎研究
- 出版年
- 2007
- 刊名
- 日本乳酸菌学会誌
- 卷
- 18
- 期
- 2
- 頁
- P 58-64
- 點閱數
- 2663
摘要
我々は、富山湾内にある採水施設で汲み上げられた深層水から、フィルター集菌し、フィルターをアジ化ナトリウム、シクロヘキシミド添加MRS寒天培地上に置床・培養することにより、増菌培養を行うことなく乳酸菌の分離を行った。この方法で、45lの深層水から25株の乳酸菌を分離した。得られた分離株から形態学的試験、生理学的試験および同定キットAPI20strepを用いて、Enterococcus faecium 8株、Enterococcus durans 2株およびEnterococcus faecalis 1株を選抜した。これらの菌株の内、E. faecium TN-3およびE. faecalis TN-9と判定された2株の16S rDNA解析では、TN-3 株はE. durans に、TN-9 株はE. faecalis に最も高い相同性を示した。また、これら2株はE. durans およびE. faecalis の基準株にそれぞれ同等の薬剤感受性を示した。TN-3 株、TN-9 株のvancomycinに対するMIC値はともに0.5μg/ml および1.0μg/ml であり、両菌株ともにvancomycin耐性遺伝子も検出されなかった。
Twenty-five strains of lactic acid bacteria which were acid producing, catalase-negative and gram-positive had been isolated from 45l of deep-seawater samples in Toyama Bay, Japan by the membrane filter method performed using the modified de Man-Rogosa-Sharpe (MRS) agar medium. Eleven strains out of them were classified as 8 of Enterococcus faecium, as 2 of Enterococcus durans and as one of Enterococcus faecalis based on morphological and physiological characteristics, together with the results of API20strep identification kit. The strains TN-3 and TN-9, which were resembled E. faecium and E. faecalis, should be identified as E. durans and E. faecalis by the 16S rDNA analyses, respectively. These 2 strains had equal antimicrobial profile for their type strain. The MIC values of TN-3 and TN-9 for vancomycin were 0.5μg/mL and 1.0μg/mL, and the vancomycin resistance genes were not detected in these 2 strains.
Twenty-five strains of lactic acid bacteria which were acid producing, catalase-negative and gram-positive had been isolated from 45l of deep-seawater samples in Toyama Bay, Japan by the membrane filter method performed using the modified de Man-Rogosa-Sharpe (MRS) agar medium. Eleven strains out of them were classified as 8 of Enterococcus faecium, as 2 of Enterococcus durans and as one of Enterococcus faecalis based on morphological and physiological characteristics, together with the results of API20strep identification kit. The strains TN-3 and TN-9, which were resembled E. faecium and E. faecalis, should be identified as E. durans and E. faecalis by the 16S rDNA analyses, respectively. These 2 strains had equal antimicrobial profile for their type strain. The MIC values of TN-3 and TN-9 for vancomycin were 0.5μg/mL and 1.0μg/mL, and the vancomycin resistance genes were not detected in these 2 strains.