培養したヒト線維芽細胞のコラーゲン合成に対する伊豆赤沢海洋深層水の効果(Effects of deep seawater pumped at Izu-Akazawa on collagen synthesis in cultured human fibroblasts)
- 標題
- 培養したヒト線維芽細胞のコラーゲン合成に対する伊豆赤沢海洋深層水の効果(Effects of deep seawater pumped at Izu-Akazawa on collagen synthesis in cultured human fibroblasts)
- 作者
- 野村 道康, 有賀 みずえ, 山田 勝久, 今田 千秋, 小林 武志, 濱田(佐藤) 奈保子(Michiyasu Nomura, Mizue Aruga, Katsuhisa Yamada)
- 文件屬性
- 日本研究
- 知識分類
- 醫療保健
- 出版年
- 2011
- 刊名
- 海洋深層水研究
- 卷
- 12
- 期
- 1
- 頁
- P11-17
- 點閱數
- 2391
摘要
コラーゲンは美容を考えるうえで重要な真皮マトリ、，クス成分のーつで，その合成にはビタミン C が必要である． 著者らは，ビタミンC の存在下で，ヒトの皮膚由来の線維芽細胞（以下，NB 1) を伊豆半島赤沢（水深800 m）から取水されている海洋深層水（以下，DSW）とその直上の表面 海水（以下，ssw）を添加した培地で培養し，コラーゲンタンパク質（以下，コラーゲン）の合 成量の違いを調べた． その結果，ssw では対照区であるssw 無添加区と比べ1.1 倍に上昇したコ ラーゲン合成量が，DSW ではDSW 無添加区と比べ1.9 倍と，ssw と比較し顕著に上昇した． さ らに，DSW添加区のNB1 ではI型コラーゲンタンパク質遺伝子（COL1) の発現が早まることが 判明した．
Collagen is an important composition of skin matrix to be paid attention in cosmetology. In the present study, effects of deep seawater on the synthesis of collagen in fibroblasts from human skins (NB1)were examined in culture. The fibroblasts were cultured in the presence of vitamin C (20ug/mL) with 0 to 20%deep seawater (DSW) pumped from a depth of 800 m (5 km offshore) and in surface seawater (SSW) collected directly at the sea surface off Akazawa in Izu Peninsula, Shizuoka Prefecture, Japan. As a result, the collagen synthesis rose to 1.1 times in SSW compared with no added SSW. In contrast, the collagen synthesis remarkably rose to 1.9 times in DSW compared with no additive DSW. Other observations made during this study suggest that DSW promotes the expression of genes involved in Type I collagen synthesis (COL1)in the early stage in fibroblasts cultured with vitamin C.
Collagen is an important composition of skin matrix to be paid attention in cosmetology. In the present study, effects of deep seawater on the synthesis of collagen in fibroblasts from human skins (NB1)were examined in culture. The fibroblasts were cultured in the presence of vitamin C (20ug/mL) with 0 to 20%deep seawater (DSW) pumped from a depth of 800 m (5 km offshore) and in surface seawater (SSW) collected directly at the sea surface off Akazawa in Izu Peninsula, Shizuoka Prefecture, Japan. As a result, the collagen synthesis rose to 1.1 times in SSW compared with no added SSW. In contrast, the collagen synthesis remarkably rose to 1.9 times in DSW compared with no additive DSW. Other observations made during this study suggest that DSW promotes the expression of genes involved in Type I collagen synthesis (COL1)in the early stage in fibroblasts cultured with vitamin C.