駿河湾の水深687mから汲み上げられた深層水から単離された珪藻Rhaphoneis crinigera(A diatom Rhaphneis crinigera Takano isolated from deep seawater from a depth of 687m in Suruga Bay)
- 標題
- 駿河湾の水深687mから汲み上げられた深層水から単離された珪藻Rhaphoneis crinigera(A diatom Rhaphneis crinigera Takano isolated from deep seawater from a depth of 687m in Suruga Bay)
- 作者
- 松浦 玲子, 花井 孝之, 岡本 一利, 石井 織葉, 鈴木 秀和(Reiko Matsuura, Takayuki Hanai, Kazutoshi Okamoto)
- 文件屬性
- 日本研究
- 知識分類
- 基礎研究
- 出版年
- 2012
- 刊名
- 海洋深層水研究
- 卷
- 13
- 期
- 1
- 頁
- P1-6
- 點閱數
- 2584
摘要
駿河湾深層水取水供給施設では，水深687m から延長約7.2 km のシームレス管を通して深層 水が供給されている． 本研究では，施設の取水口から汲み上げた駿河湾深層水から直接採水した後 直ちに密閉し，細心の注意を払いながら培養を行った結果，これまで国内浅海域で報告されていた 海産浮遊珪藻Rhaphoneis crinigera Takano を単離することができた． これまでにも海洋深層水 取水施設から多くの種の珪藻の出現が報告されているが，取水後の混入の可能性もあった．従って， 今回の報告は，深層水由来であることが‘最も確実、で，R. crinigeta の最深採集記録であるだけ でなく，深層水取水管を通して採集されたすべての珪藻の最深採集記録である． 本種はろ過滅菌し た駿河湾深層水のみで7 年以上継代培養が可能であった．
At some domestic deep seawater (DSW) utilization facilities, occurrence of diatom has been reported from aquaria or tap DSW. As previous studies were aimed to make in si飢 diatom flora in the facilities as databases for further use, little efforts have been made to separate species contaminated after being pumped on land. In the present study, we carefully sampled a bottle of DSW pumped from Suruga Bay, central Pacific coast of Honshu, Japan, and isolated one diatom species of highly possible DSW origin. The DSW sample (700 ml) was immediately packed after tapped through a 7,200 m long seamless pipeline deployed from a depth of 687 m off Yaizu City. After culturing at a condition (20C, 25 1umol/m2/sec, 12L:12D) for four months (three month in still water and one month on agar plate) using filtered and sterilized DSW, a diatom was isolated among the colony of microorganisms. The diatom was identified as a planktonic pennate species, Rhaphoneis crinigera Takano by mor-phological observation using the light and scanning and transmission electron microscops. This is not only the deepest record of R. crinigera but that among all of the diatoms collected through pipelines. Surprisingly, the diatom could be passaged 79 times during 7 years by changing with filtered and sterilized DSW once a month.
At some domestic deep seawater (DSW) utilization facilities, occurrence of diatom has been reported from aquaria or tap DSW. As previous studies were aimed to make in si飢 diatom flora in the facilities as databases for further use, little efforts have been made to separate species contaminated after being pumped on land. In the present study, we carefully sampled a bottle of DSW pumped from Suruga Bay, central Pacific coast of Honshu, Japan, and isolated one diatom species of highly possible DSW origin. The DSW sample (700 ml) was immediately packed after tapped through a 7,200 m long seamless pipeline deployed from a depth of 687 m off Yaizu City. After culturing at a condition (20C, 25 1umol/m2/sec, 12L:12D) for four months (three month in still water and one month on agar plate) using filtered and sterilized DSW, a diatom was isolated among the colony of microorganisms. The diatom was identified as a planktonic pennate species, Rhaphoneis crinigera Takano by mor-phological observation using the light and scanning and transmission electron microscops. This is not only the deepest record of R. crinigera but that among all of the diatoms collected through pipelines. Surprisingly, the diatom could be passaged 79 times during 7 years by changing with filtered and sterilized DSW once a month.