DGGE 法による海洋深層水中の放線菌の群集組成解析(Community Analysis of Actinomycetes in Deep Seawater using Denaturing Gradient Gel Electrophoresis(DGGE))
- 標題
- DGGE 法による海洋深層水中の放線菌の群集組成解析(Community Analysis of Actinomycetes in Deep Seawater using Denaturing Gradient Gel Electrophoresis(DGGE))
- 作者
- 寺原 猛, 加藤 朋, 山田 勝久, 小林 武志, 今田 千秋(Takeshi Terahara, Tomo Kato, Katsuhisa Yamada)
- 文件屬性
- 日本研究
- 知識分類
- 基礎研究
- 出版年
- 2013
- 刊名
- 海洋深層水研究
- 卷
- 14
- 期
- 1
- 頁
- P11-18
- 點閱數
- 2662
摘要
低温，高水圧の特異環境下から汲み上げられた海洋深層水（DSW）は，応用微生物学的見地から も様々な利用が期待されるが，微生物に関する基礎的知見は乏しいのが現状である． そこで，本研 究ではDSW 中に存在する微生物の中から，諸産業において重要視されている放線菌に着目し，変 性剤濃度勾配ゲル電気泳動法（DGGE法）を用いて，伊豆赤沢・焼津沖（駿河湾）・室戸沖のDSW と表面海水（SSW）の放線菌の群集組成を調べた． その結果，DSW とssw では，放線菌の群集組 成に大きな相違が確認された． また，伊豆赤沢の群集組成を多次元尺度法（MDS) で解析したとこ ろ，SSWでは放線菌の群集組成は大きく変化し，季節ごと・（3月一5月，6月一8月，9月一11 月，12月一1月）に異なった群集を形成している傾向があったが，DSWでは群集組成はsswと 比較して変化が少なく． 年間を通じて安定していることが示唆された．
Deep seawater (DSW) pumped up from unique environments such as those with low tempera-ture and high pressure is of interest as excellent sources of beneficial microorganisms; however, only a few studies have been undertaken to investigate microbial community in DSW. In this study, we examined community structures of actinomycetes in DSW and surface seawater (SSW) samples collected from three DSW pumping stations in Japan (Izu-Akazawa and Yaizu in Shizuoka Prefecture and Muroto in Kochi Prefecture). Denaturing gradient gel electrophoresis (DGGE) analyses revealed that the actinomycete community structures were markedly different between DSW and SSW samples. In addition, multi-dimensional scaling (MDS) analysis of the obtained DGGE profiles in Izu-Akazawa indicated that the actinomycete community structures in SSW varied seasonally and tended to be uniquely different structures in each season, whereas those in DSW were stable throughout the year compared with the structures in SSW.
Deep seawater (DSW) pumped up from unique environments such as those with low tempera-ture and high pressure is of interest as excellent sources of beneficial microorganisms; however, only a few studies have been undertaken to investigate microbial community in DSW. In this study, we examined community structures of actinomycetes in DSW and surface seawater (SSW) samples collected from three DSW pumping stations in Japan (Izu-Akazawa and Yaizu in Shizuoka Prefecture and Muroto in Kochi Prefecture). Denaturing gradient gel electrophoresis (DGGE) analyses revealed that the actinomycete community structures were markedly different between DSW and SSW samples. In addition, multi-dimensional scaling (MDS) analysis of the obtained DGGE profiles in Izu-Akazawa indicated that the actinomycete community structures in SSW varied seasonally and tended to be uniquely different structures in each season, whereas those in DSW were stable throughout the year compared with the structures in SSW.