DGGE法による海洋深層水中の微生物群集組成解析(Microbial Community Analysis of Deep Seawater by Denaturing Gradient Gel Electrophoresis)
- 標題
- DGGE法による海洋深層水中の微生物群集組成解析(Microbial Community Analysis of Deep Seawater by Denaturing Gradient Gel Electrophoresis)
- 作者
- 寺原 猛・山口貴大・山田勝久・小林武志・今田千秋(Takeshi Terahara, Takahiro Yamaguchi, Katsuhisa Yamada, Takeshi Kobayashi And Chiaki Imada)
- 文件屬性
- 日本研究
- 知識分類
- 水產養殖
- 出版年
- 2014
- 刊名
- 海洋深層水研究
- 卷
- 15
- 期
- 1
- 頁
- P11-17
- 點閱數
- 2375
摘要
自然界でも微生物に関する研究が少ない海洋深層水（DSW）中には全く未知の，あるいは特異 的な生理・生化学的諸性状を有する微生物の存在が期待されるが，微生物に関する基礎的知見は 乏しいのが現状である． そこで，本研究ではDSW中に存在する微生物の中から，一般細菌および 真菌を対象にして，変性剤濃度勾配ゲル電気泳動法（DGGE法）を用いて，微生物群集組成をそれ ぞれ調べた． その結果，年間を通じて様々な群集の存在が示唆された． また，溶存態有機物に付 着している微生物（A）と遊離して存在している微生物（F) の群集組成は異なっていることがわか った． さらに，多次元尺度法（MDS法）で解析した結果，一般細菌および真菌ともに，群集組成 は変遷することが示唆された． また，DGGEのバンドをシーケンス解析し，微生物を同定した結 果，DSW中には属レベルで新規と思われる細菌や新規性が高いと思われる真菌の存在が示唆され た．
Deep seawater(DSW), about which there are few microbial reports, is of interest as excellent sources of novel microorganisms or ones with unique natures; however, only a few studies have been undertaken to investigate the microbial community in DSW In this study, we examined the community structures of bacteria and fungi in DSW samples collected from the pumping stations for DSW in Izu-Akazawa in Shizuoka Prefecture by denaturing gradient gel electrophoresis (DGGE) analyses. DGGE analyses revealed that several kinds of bacteria and fungi were present in DSW and the microbial community structures of attached microbes and free-living microbes differed. In addition, multi-dimensional scaling (1VIDS) analysis of the obtained DGGE profiles in-dicated that each community structure of bacteria and fungi changed throughout the year with the exception of a few months. Furthermore, sequence analyses of the obtained DGGE profiles to identify microorganisms indicated that bacteria belonging to new genera and novel fungi were present in DSW samples collected from the pumping stations in Izu-Akazawa.
Deep seawater(DSW), about which there are few microbial reports, is of interest as excellent sources of novel microorganisms or ones with unique natures; however, only a few studies have been undertaken to investigate the microbial community in DSW In this study, we examined the community structures of bacteria and fungi in DSW samples collected from the pumping stations for DSW in Izu-Akazawa in Shizuoka Prefecture by denaturing gradient gel electrophoresis (DGGE) analyses. DGGE analyses revealed that several kinds of bacteria and fungi were present in DSW and the microbial community structures of attached microbes and free-living microbes differed. In addition, multi-dimensional scaling (1VIDS) analysis of the obtained DGGE profiles in-dicated that each community structure of bacteria and fungi changed throughout the year with the exception of a few months. Furthermore, sequence analyses of the obtained DGGE profiles to identify microorganisms indicated that bacteria belonging to new genera and novel fungi were present in DSW samples collected from the pumping stations in Izu-Akazawa.